OPEN-ACCESS PEER-REVIEWED
1Ms Jhansirani Ganapa, 2Dr. Dasu Naidu Kuna, 3Mr. Sudheer Choudari
1Assistant Professor, Department of Computer Science and Engineering Centurion University of Technology and Management, Vizianagaram, Andhra Pradesh.
2Assistant Professor, Department of BS&H, GMR Institute of Technology.
3Assistant Professor, Civil Engineering Department, Centurion University of Technology and Management, Andhra
Abstract:
Bioluminescent imaging (BLI) has emerged as a powerful tool for longitudinal tracking of transplanted stem cells in regenerative medicine. This review discusses the historical evolution of BLI technology and key innovations that have expanded its sensitivity and resolution for imaging cellular processes in living subjects. Current strategies for engineered bioluminescent reporters to enhance signal strength are also covered, including promoter selection, codon optimization, and fusion protein designs tailored to stem cells. The review highlights BLI applications in disease models across various organ systems, particularly for monitoring stem cell engraftment, migration, proliferation and differentiation dynamics. Ongoing trends focus on coupling BLI with therapies and tissue engineering scaffolds for advancing precision medicine. Overall, BLI enables non-invasive monitoring of stem cell fate in vivo, which is instrumental for clinical translation.
Keywords: Bioluminescence imaging, stem cells, regenerative medicine, in vivo tracking, luciferase reporters
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