OPEN-ACCESS PEER-REVIEWED
1Rishabh Rana, 2Romita Sharma, 3Anand Kumar, 4Saurabh Kumar
1Cancer Biology, Microbiology, Department of Biotechnology, Shoolini University
2(Cancer Biology), Department of Biotechnology, Shoolini University
3(Biotechnology), Department of Pharmaceutical Science, Shoolini University,
4(Microbiology), Department of Biotechnology, Shoolini University
Abstract
This review explores advancements in bioanalytical research, specifically focusing on Ultra High-Performance Liquid Chromatography-Mass Spectrometry (UHPLC-MS). The study details a refined analytical procedure for quantifying catechin derivatives in tea samples, combining optimized infusion preparation with validated UHPLC–MS/MS methods. It delves into challenges of bioanalytical method development, including flawed extraction techniques, analytical issues in HPLC and LC-MS/MS, internal standard selection, reporting intricacies, and sample shipping complexities. Emphasizing the role of sponsors, the article discusses implications for future bioanalytical research. Titled “Advances in Ultra HPLC-MS: Unraveling the Bioanalytical Landscape of Drug Detection and Metabolite Profiling,” the review urges ongoing methodological advancements and adherence to Good Laboratory Practices (GLP) and Good Clinical Practices (GCP).
Keywords: UHPLC-MS, Catechin quantification, HPLC, LC-MS/MS, Reporting issues, Sample shipping, Drug detection, Metabolite profiling
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