A Study On Quantitative Estimation Of Secondary Metabolites And In Vitro Dipeptidyl Peptidase IV (DPP-IV) Inhibitory Activity Of Coccinia Grandis Fruit Extract.
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Abstract
Nature provides an abundant source of medicines that can be used to treat diabetes. A range secondary metabolites, including alkaloids, terpenoids and flavonoids have shown notable anti-diabetic effects. These natural compounds can increase insulin secretion, boost insulin sensitivity, and lower blood glucose levels. Coccinia grandis (ivy gourd) have demonstrated promising potential in the field of medicine due to their rich phytochemical composition. Various cultural settings have traditionally utilized these herbs for their purported therapeutic properties, particularly in the field of diabetes management.
Objective: The present study objective is to investigate the antidiabetic potential of Hydroalcoholic extract Coccinia grandis fruit through in vitro assays with DPP-IV enzyme inhibition and quantitative estimation with standard producers.
Materials and Methods: The current research investigated DPP-IV inhibitory mechanism of C.Grandis fruit extract by utilizing the spectrophotometric approach and Sitagliptin serves as a positive control. The efficacy of inhibition has been evaluated by percent inhibition.
Results: The findings indicated that the extract effectively suppressed DPP-IV activity, comparable to sitagliptin but at a concentration that was ten times greater. The extract exhibited IC50 value of 88.22μg/mL, whereas the IC50 value of sitagliptin was 44.68μg/ml. The total flavonoid, phenolic, and alkaloid content in the fruit of coccinia grandis was measured to be 3.15 mg/100mg, 2.24mg/100mg, and 2.14 mg/100 respectively.
Conclusion: the results indicate that the Hydroalcoholic extract of coccinia grandis has higher flavonoid content compared to alkaloids and phenols which may have a potential DPP-IV inhibitory effect that can serve potential anti-diabetic agent.
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