OPEN-ACCESS PEER-REVIEWED

1Sudheer Choudari, 2Dr. L Subha, 3RASHMI N, 4Dr. Mukesh Kumar Chandrakar, 5Thangavel Pradeeshkumar, 6Anu Singh,. 7Harmohan Singh Yadav

1Assistant Professor,Deaprtment of Civil Engineering, Centurion University of Technology and Management, Andhra Pradesh

2Assistant professor (Plant Breeding and Genetics) Tamilnadu Agricultural University, Agricultural Research Station, Pattukkottai Thanjavur, Tamilnadu

3Assistant Professor Department of Electronics and Communication Engineering BMS Institute of Technology and Management, India

4Assistant Professor, Department of Electronics and Telecommunication Engineering, Bhilai Institute of Technology Durg,Chhattisgarh, India

5Department of Agronomy VIT School of Agricultural Innovations and Advanced Learning (VAIAL), VIT, Vellore 632 014, Tamil Nadu, India

6Department of  microbiology Swami Vivekanand University, Sagar, M.P., India

7Associate Professor Department of soil science and agricultural chemistry, School of Agriculture, Lovely Professional University Jalandhar Punjab, India 144411

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Abstract

Foodborne illnesses caused by microbial pathogens present a major public health concern worldwide. Conventional methods for detection of foodborne pathogens are time- consuming and labor-intensive. Thus, there is a need for rapid, sensitive, and specific techniques to screen agricultural products for microbial contamination. This research aims to develop optical biosensors for quick on-site detection of common foodborne pathogens such as Salmonella, Listeria monocytogenes, and Escherichia coli O157:H7 in fresh produce and animal products. The biosensors will utilize immobilized antibodies or aptamers that specifically bind to target pathogens. Pathogen binding will be detected by changes in optical signals from fluorescent labels, gold nanoparticles, or other transducers. Various optical detection modes will be explored, including fluorescence, absorbance, surface plasmon resonance and refractive index measurement. The optical biosensors will be incorporated into portable devices or disposable test strips. The performance of the biosensors will be evaluated in pure cultures and naturally contaminated foods. Successful development of rapid optical biosensors could help prevent outbreaks of foodborne illness associated with consumption of contaminated agricultural commodities. The biosensor technology also has broad applicability for detection of microbial hazards and spoilage organisms in the food industry.

Keywords: Foodborne, biosensors, contaminated, agricultural, aptamers, optical, signals

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